1. What is PZLAST?

PZLAST is an ultra-fast amino acid sequence similarity search service.

Using PZLAST, users can search the protein sequences to several Tera-bytes of public metagenomic amino acid sequences in approximately 10-20 minutes.

PZLAST uses PEZY-SC2, which is a MIMD-type many-core processor [1][2][3][4]. The algorithm of PZLAST is basically similar to CLAST [5].


PEZY-SC2 (in English)
[1] ZettaScaler: Liquid immersion cooling Manycore based Supercomputer
[2] pzqd: PEZY-SC2 Acceleration of Double-Double Precision Arithmetic Library for High-Precision BLAS

PEZY-SC2 (in Japanese)
[3] PEZY Computing: PEZY-SC2モジュール&プロセッサ
[4] グリーンスーパーコンピュータ ZettaScaler の技術と今後の展望

[5] CLAST: CUDA implemented large-scale alignment search tool


If you find PZLAST useful in your research, please cite the following paper:

Hiroshi Mori, Hitoshi Ishikawa, Koichi Higashi, Yoshiaki Kato, Toshikazu Ebisuzaki, Ken Kurokawa, PZLAST: an ultra-fast amino acid sequence similarity search server against public metagenomes, Bioinformatics, 2021;, btab492, https://doi.org/10.1093/bioinformatics/btab492

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3. Contact.

This web site is operated by Genome Evolution Laboratory at the National Institute of Genetics and Computational Astrophysics Laboratory at RIKEN.
If you have any question, request, or suggestion, please contact the E-mail address below.

4. Releases.

v. 0.3.1 (2022-07-20)
Added "Sample-wise results" to the results page.
Completion-table, -MEO cloud, -world map results are organized into the "Sample-wise results" tab.

v. 0.3.0 (2022-07-13)
Added two features to the results page.
One is environmental coverage information for each amino acid residue, and the other is a table of query hit fulfillment rates for each sample.

v. 0.2.1 (2021-12-17)
Reference database has been updated.
As the number of reference sequences increased from 42 Billion to 64 Billion, the processing time of the search increased by about 20%.

v. 0.2.0 (2021-8-20)
Alignment information has been added to the output table.

v. 0.1.0 (2020-4-1)
PZLAST Web Service: First release.